Human Fibroblast (hFIB) Reprogramming Kit

Description

Stemmera^TM Human Fibroblast (hFIB) Reprogramming Kit is a ready-to-use and fully chemically defined reprogramming kit which composed of necessary reagents and tools intended for generation of human induced pluripotent stem cells (iPSCs) from fibroblast and maintenance of iPSCs under completely serum-free and feeder-free conditions.

Features

• Ready-to-use for human fibroblast culture, human fibroblast reprogramming and freezing of iPSCs
• One-step reprogramming
• High efficiency reprogramming
• Serum/feeder-free culture and maintenance
• Easy protocol

Product Component

Human Fibroblast Reprogramming Kit-I

• Cat# ST01003-S1 0.2% Gelatin (50ml)
• Cat# ST01003-S2/BM Fibroblast Growth Basal Medium (225ml)
• Cat# ST01003-S3 1x PBS (50ml)
• Cat# ST01003-S4 TrypLE (25ml)
• Cat# ST01003-S7 Non-Enzymatic Cell Dissociation Solution (100ml)
• Cat# ST01003-S8/BM Fibroblast Reprogramming Basal Medium (2x500ml)
• Cat# ST01003-S13 DMEM/F12 (60ml)

Human Fibroblast Reprogramming Kit-II

• Cat# ST01003-S2/S Fibroblast Growth Supplement (25ml)
• Cat# ST01003-S6 Polybreen (10μl)
• Cat# ST01003-S8/S Fibroblast Reprogramming Supplement (2×3.5ml)
• Cat# ST01003-S9 Matrix (5×100μl)
• Cat# ST01003-S10 Rock Inhibitor (500μl)
• Cat# ST01003-S14 Fibroblast Cryopreservation Solution (15ml)
• Cat# ST01003-S15 Serum-Free Cryopreservation Solution for hPSC (20ml)

Human Fibroblast Reprogramming Kit-III

• Cat# ST01003-S11-24 Cell Culture Plates (1×24 well)
• Cat# ST01003-S11-12 Cell Culture Plates (1×12 well)
• Cat# ST01003-S11-6 Cell Culture Plates (4×6 well)
• Cat# ST01003-S12 Cell Counter Slides (3pcs)
• Cat# ST01003-S5 Trypan Blue (300μl)

Reprogramming Factors (Not supplied)

Note: Sendai virus reprogramming factors from Life Technologies Inc is strongly recommended, Cat # A16517

Storage & Handling
Store all components as directed in manual instruction upon arrival and until the expiration dates stated on the product label. Avoid multiple freeze/thaw cycles. Make aliquot of complete medium for daily use, avoid warm up cycles. Protect from light is preferred.

Product Use
This product is intended for in vitro use and research use only. Not intended for human or animal diagnostic or therapeutic uses.