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Human Blood Cell Reprogramming Kit

Human Blood Cell (hBLD) Reprogramming Kit

For induction of human iPSCs from Peripheral Blood Mononuclear Cells (PBMCs)

ProductCatalog#SizePricing
Human Blood Cell (hBLD) Reprogramming Kit ST01005 5 reactions/kit $2250.00

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Description
StemmeraTM Human Blood Cell (hBLD) Reprogramming Kit is a ready-to-use and fully chemically defined reprogramming kit which composed of necessary reagents and tools intended for culturing human PBMCs and induction of pluripotent stem cells (hiPSCs) from PBMCs, as well as maintenance of iPSCs under completely serum-free and feeder-free conditions.

Features

  • Ready-to-use for blood cell culture, blood cell reprogramming & freezing of iPSCs
  • One-step reprogramming
  • High efficiency reprogramming
  • Serum/feeder-free culture and maintenance
  • Easy protocol

Product Component

Human Blood Cell Reprogramming Kit-I

  • Cat# ST01005-S1 Non-Enzymatic Cell Dissociation Solution (100ml)
  • Cat# ST01005-S2/BM Blood Cell Reprogramming Basal Medium (2x500ml)
  • Cat# ST01005-S3 DMEM/F12 (60ml)

Human Blood Cell Reprogramming Kit-II

  • Cat# ST01005-S4/BM Blood Cell Basal Medium (2x25ml)
  • Cat# ST01005-S4/S Blood Cell Supplement (61μl for 25ml of Basal Medium)
  • Cat# ST01005-S5 Polybreen (10μl)
  • Cat# ST01005-S6 Matrix (5x100μl)
  • Cat# ST01005-S2/S Blood Cell Reprogramming Supplement (2x3.5ml)
  • Cat# ST01005-S7 Rock Inhibitor (1000x) (500μl)
  • Cat# ST01005-S8 Blood Cell CryopreservationSolution (10ml)
  • Cat# ST01005-S9 Serum-Free Cryopreservation Solution for hPSC (20ml)

Human Blood Cell Reprogramming Kit-III

  • Cat# ST01005-S10-24 Cell Culture Plates (1x24 well)
  • Cat# ST01005-S10-12 Cell Culture Plates(1x12 well)
  • Cat# ST01005-S10-6 Cell Culture Plates(4x6 well)
  • Cat# ST01005-S11 Cell Counter Slides (3pcs)
  • Cat# ST01005-S12 Trypan Blue (300μl)
  • Reprogramming Factors (Not supplied)

Note: Sendai virus reprogramming factors from Life Technologies Inc is strongly recommended, Cat # A16517

Storage & Handling
Store all components as directed in manual instruction upon arrival and until the expiration dates stated on the product label.Avoid multiple freeze/thaw cycles. The complete medium should remain stable for 4-6 weeks at 2-8°C. Make aliquot of complete medium for daily use, avoid warm up cycles. Protect from light is preferred.

Product Use
This product is intended for in vitro use and research use only. Not intended for human or animal diagnostic or therapeutic uses.

 
Product NameDocument TypeDownload
Human Blood Cell (hBLD) Reprogramming Kit Manual PDF icon
Human Blood Cell (hBLD) Reprogramming Kit Datasheet PDF icon
Blood Cell Basal Medium & Supplement MSDS PDF icon
Blood Cell Cryopreservation Solution MSDS PDF icon
Blood Cell Reprogramming Basal Medium & Supplement MSDS PDF icon
DMEM-F12 MSDS PDF icon
Matrix MSDS PDF icon
Non-Enzymatic Cell Dissociation Solution MSDS PDF icon
Polybrene MSDS PDF icon
Rock Inhibitor MSDS PDF icon
Serum-Free Cryopreservation Solution for hPSC MSDS PDF icon
Trypan Blue MSDS PDF icon
 

Quality Control
This product is used to reprogram human PBMCs to iPSCs and maintain the pluripotency of human iPSCs under serum-/feeder-free culture condition. To ensure the quality, the following images represent human PBMCs in suspension and the pluripotent morphology of human iPSCs growing on Matrigel with StemmeraTM Human Blood Cell (hBLD) Reprogramming Medium.

 

Welcome!

Stemmera Inc is a privately owned biotechnology company located in Bay Area, California that focus in developing innovative technology and application solution for stem cell research. Our products include kit, culture medium, reagents, antibodies, buffers and solution, cell lines that are convenient tools used in stem cell research. Read More